human body back muscles

In my recent quest to find new, cutting-edge research on cephalopods, Ive come across some neat stuff (check out this post on the perception of polarized light by cuttlefish - its one of my favorite new cephalopod research topics!)  The study Ill review here is outside of my field of relative expertise, but its so neat and so new that I couldnt resist writing about it.  Its good to step out of ones comfort zone every once in a while, right?

An international team of researchers hailing from Norway, Australia, and Germany has published a study on the venom of Antarctic octopods (more accurately, it is being published, though it hasnt hit the presses yet.)  The team investigated the biochemical properties of extracts from the salivary gland of four Antarctica octopus species and wrote up their results in Venom on ice: First insights into Antarctic octopus venoms (2010).

Here is their image of the posterior salivary glands of an octopus, from which the authors collected all of their specimens:
These glands produce a variety of compounds, notably venom and digestive enzymes.  The venom of temperate-water octopuses has been studied in the past.  Never before, however, has venom been studied in an octopus that lives in below-freezing temperatures, conditions under which the enzymes in most venoms work very poorly if at all.  To begin to understand the role of venoms in the lives of Antarctic octopuses, the team collected and tested venom from four octopus species collected off the Antarctic shore: Adelieledone polymorpha, Megaleledone setebos, Pareledone aequipapillae, and Pareledone turqueti.  Here are images of some of their specimens:



Cute, arent they?  Octopods always are!  Anyways, back to the biochemical assays.

First, the authors tested the extracts for alkaline phosphatase (ALP) activity.  ALP is an enzyme that is in spider and snake venom that is thought to help immobilize prey items.  Second, they tested for Acetylcholinesterase (AChE) activity.  AChE breaks down acetylcholine, a neurotransmitter, potentially acting as a toxin by disrupting neuromuscular function.  Third, the extracts were tested for general proteolytic activity using casein.  Fourth, an assay for secreted phospholipase A2 (sPLA2) was performed.  sPLA2 is found in cone snail and snake venome, and contributes to the effects of venoms in a variety of ways.  Finally, the researches assessed whether the venoms showed haemolytic activity, which is a common marker of the general toxic activity of venoms.  Taken together, these results should begin to characterize the putative venom of each octopus species.  After all of this, the researchers reviewed what is known about the morphology of the mouthparts of the octopuses, as well as their feeding habits, and tried to relate these to their biochemical findings.

Whew.

So, after all of that, what did they find?  Here are their results(takes another deep breath):

Venom from all of the species had some ALP activity.  Interestingly, however, when ALP activity was tested at 0 Celsius and at 37 Celsius, venom from 3 species of octopus (A. polymorpha, M. setebos, and P. turqueti) had higher ALP activity at the lower temperature!  This is a significant finding because it suggests some sort of modification of the proteins responsible for this activity to function optimally at a lower temperature.  This lends some weight to the theory that the use of venom has been important enough to the survival of Antarctic octopus species that they have evolved enzymes to work under conditions where most enzymatic toxins would not.  In the other tests, an essentially similar pattern of results were found, except for the AChA activity assay.  Little AChA activity was found in any of the species, although the results of the assay were poor enough (that is, inconsistent) that they were not included in the paper.  Interestingly enough, although all of the species had a few potentially functional toxins in their venom, most of them showed only weak haemolytic activity.  Only one extract (from P. turqueti) showed strong haemolytic activity.

The relation of venom activity to morphology and diet that the authors attempted to point out appears to be weak (or at least difficult to point out given their sample,) as it is mentioned that few clear venom-related adaptations in diet or anatomy were present in these octopus species.  A. polymorpha is noted to have a very large salival gland and a narrow beak, which the authors suggest might be an adaptation associated with the use of venom as a primary means of catching prey (as opposed to having powerful jaws to physically overpower the prey.)  This species feeds mostly on amphipods and polychaete worms, and so its unclear why it would rely on venom to subdue such (relatively) easy going prey instead of retaining a more varied diet.  In any case, though, this is one of those papers that, being exploratory, raises many more questions than it answers - thats the kind I like!

What I find most interesting about this work is that it begs questions about the evolution of octopus venom.  How quickly could the octopus populations move into cold water?  Was this limited by the evolution of venom enzymes, or did that evolution occur after some quicker relocation of the species which left their warm-water-adapted enzymes useless?  Did A. polymorphas ancestors have a specialized diet before they became Antarctic, or is that only a successful feeding strategy in the Antarctic environment?  The world may never know (although I hope we do, someday!)

Thanks for reading!


Undheim, E.A.B., et al. (2010). Venom on Ice: First insights into Antarctic octopus venoms Toxicon

"Our birth is nothing but our death begun, as tapers waste the moment they take fire."--Young.

The arrival of the stork with the new baby is an event of vast family interest, especially if it is the first visit of the bird to the domicile. In America it is not customary to announce a birth in the newspapers, as is often done in England, especially among the nobility. The personal friends of the parents receive the visiting card of both, or of the mother only, to which is attached a small card bearing the babys full name and the date of his arrival. These are enclosed in an envelope, this again in an outer one, and mailed.
It is proper for those thus notified to call at an early date to in¬quire as to the well-being of mother and babe. As it is not customary for the mother to receive any but a very few of her nearest relatives under at least three weeks, callers should not be expected to see her, but are to leave cards. A note of congratulation is often sent instead of calling, and offers to the ingenious and witty an excellent chance for the display of delicate pleasantry. Thus it is entirely proper to ad¬dress the note to the baby, and congratulate him on having chosen such charming parents, and such a lovely home. Flowers are not infrequently sent to the mother, and little gifts--soft booties, little gold pins for sleeve and neck, little crocheted or knitted sacks, or dainty bibs--to the baby.

Christening Ceremony

The baby is usually christened when it is six or eight weeks old. Clergymen prefer this should be done at the church, and generally arrange to perform several baptisms at the same time--¬Childrens Day being a favorite time. Otherwise, the christening usu¬ally takes place after the congregation is dismissed at the conclusion of a service. Only those interested and a few specially invited friends remain for it. There is no objection, however, to having a child christened at home, when the affair is made one of more festivity.
Most young married people prefer to have the clergyman who married them christen their first baby, when practicable.

Sponsors

The babys sponsors are chosen, by the parents agreement, from among their relatives and close friends, almost always those of their own communion. The request is preferred verbally or by personal notes. A boy has a godmother and two godfathers; a girl two godmothers and a godfather. Occasionally this rule is broken and a godmother alone chosen for a girl, and one godfather for a boy. Godparents are supposed to stand in a more intimate relation to their godchildren than to others, and to take a more personal interest in them, especially in case of the parents death. It is a serious relation, involving a certain religious responsibility, and is not to be lightly entered into.
The godparents are expected to make christening gifts to the child on his baptismal day. They are usually in the form of silver cups, porringers, silver spoons, forks, etc.; these should be solid, never plated ware. If the babe is named for one of its godparents, the latter is expected to do something handsome in the way of a christening gift. Sometimes a bank account is opened in the childs name, the sum deposited being left at interest until he becomes of age.

Church Christenings

At a church christening, the babe is dressed in its handsomest robe and cap. Formerly the robes were very long and miracles of lace and embroidery; at present the finest of linen lawn or batiste, with a little real lace at neck and sleeves, and a bit of fine French embroidery, is thought in better taste, even in the case of the very wealthy. And many a blessed baby is given his name in a simple little lawn robe with no embellishment beyond a little tucking¬--done by the mothers own hands, perhaps.
The nurse carries the child into the church. Sponsors and parents group themselves around the font, which is often decorated with white flowers. The godmother has the privilege of holding the babe until it is time to lay him in the clergymans arms, the cap having been removed. The parents make the responses; after the naming the godmother takes the little one again, holding him until the close of the service. She should not wipe away any of the water placed on the childs head. A good baby is expected not to cry during the ceremony, and one advantage of an early christening is that the little fellow is less liable to be alarmed at strange surroundings.
The same forms are observed at a home christening, the hour being usually in the afternoon.
A luncheon to which the clergyman and the christening party, and a few friends if desired, are invited, customarily follows the church ceremony--unless several children of other families are baptized at the same time--and always follows the home christening. It is not unusual to make some recognition of a clergymans services at a church christening, and always is in order at the home rite, though it is not expected as a clergyman counts on his wedding fee.
If church or house is decorated for a christening, white flowers only are employed, in conjunction with palms and ferns to relieve them. White lilies are particularly beautiful. The table is adorned with white flowers; the cakes and bonbons are white. Any desired refreshments may be served, those for afternoon tea being suitable. That old-fashioned beverage known as caudle is never served at any other time. It is dispensed in bouillon cups.

MANNERS AND SOCIAL CUSTOMS FOR OUR GREAT MIDDLE CLASS
AS WELL AS OUR BEST SOCIETY
Correspondence, Cards and Introductions, Dress for Different Occasions, Weddings, Christenings, Funerals, Etc.,
Social Functions, Dinners, Luncheons.
Gifts, "Showers," Calls, and Hundreds of Other Essential Subjects so Vital to Culture and Refinement of Men, Women, School-Girls and Boys at Home and in Public.
By MRS ELIZABETH JOHNSTONE
Excerpt from the book:
MOTHERS  REMEDIES
Over One Thousand Tried and Tested Remedies from Mothers of the United States and Canada.
By DR. T. J. RITTER
PUBLISHED BY G.H. FOOTE  PUB. CO. DETROIT MICH 1921

After the voluntary abdication of Œdipus, his two sons, Eteocles and Polynices, took possession of the crown and reigned over the city of Thebes. But Eteocles, being an ambitious prince, soon seized the reins of government himself, and expelled his brother from the throne.

Polynices now repaired to Argos, where he arrived in the dead of night. Outside the gates of the royal palace he encountered Tydeus, the son of Œneus, king of Calydon. Having accidentally killed a relative in the chase, Tydeus was also a fugitive; but being mistaken by Polynices in the darkness for an enemy, a quarrel ensued, which might have ended fatally, had not king Adrastus, aroused by the clamour, appeared on the scene and parted the combatants.

By the light of the torches borne by his attendants Adrastus observed, to his surprise, that on the shield of Polynices a lion was depicted, and on that of Tydeus a boar. The former bore this insignia in honour of the renowned hero Heracles, the latter in memory of the famous Calydonian boar-hunt. This circumstance reminded the king of an extraordinary oracular prediction concerning his two beautiful daughters, Argia and Deipyle, which was to the effect that he would give them in marriage to a lion and a boar. Hailing with delight what he regarded as an auspicious solution of the mysterious prophecy, he invited the strangers into his palace; and when he heard their history, and had convinced himself that they were of noble birth, he bestowed upon Polynices his beautiful daughter Argia, and upon Tydeus the fair Deipyle, promising at the same time that he would assist both his sons-in-law to regain their rightful patrimony.

The first care of Adrastus was to aid Polynices in regaining possession of his lawful share in the government of Thebes. He accordingly invited the most powerful chiefs in his kingdom to join in the expedition, all of whom readily obeyed the call with the exception of the kings brother-in-law, Amphiaraus, the seer. As he foresaw a disastrous termination to the enterprise, and knew that not one of the heroes, save Adrastus himself, would return alive, he earnestly dissuaded the king from carrying out his project, and declined to take any part in the undertaking. But Adrastus, seconded by Polynices and Tydeus, was obstinately bent on the achievement of his purpose, and Amphiaraus, in order to escape from their importunities, concealed himself in a hiding-place known only to his wife Eriphyle.

Now on the occasion of the marriage of Amphiaraus it had been agreed, that if he ever differed in opinion with the king, his wife should decide the question. As the presence of Amphiaraus was indispensable to the success of the undertaking, and, moreover, as Adrastus would not enter upon it without "the eye of the army," as he called his brother-in-law, Polynices, bent on securing his services, determined to bribe Eriphyle to use her influence with her husband and to decide the question in accordance with his wishes. He bethought himself of the beautiful necklace of Harmonia, wife of Cadmus, which he had brought with him in his flight from Thebes. Without loss of time he presented himself before the wife of Amphiaraus, and held up to her admiring gaze the glittering bauble, promising that if she revealed the hiding-place of her husband and induced him to join the expedition, the necklace should be hers. Eriphyle, unable to withstand the tempting bait, accepted the bribe, and thus Amphiaraus was compelled to join the army. But before leaving his home he extorted a solemn promise from his son Alcmæon that, should he perish on the field of battle, he would avenge his death on his mother, the perfidious Eriphyle.

Seven leaders were now chosen, each at the head of a separate detachment of troops. These were Adrastus the king, his two brothers Hippomedon and Parthenopæus, Capaneus his nephew, Polynices and Tydeus, and Amphiaraus.
When the army was collected they set out for Nemea, which was at this time governed by king Lycurgus. Here the Argives, being short of water, halted on the outskirts of a forest in order to search for a spring, when they saw a majestic and beautiful woman seated on the trunk of a tree, nursing an infant. They concluded from her noble and queenly appearance that she must be a goddess, but were informed by her that she was Hypsipile, queen of the Lemnians, who had been carried away captive by pirates, and sold as a slave to king Lycurgus, and that she was now acting as nurse to his infant son. When the warriors told her that they were in search of water, she laid the child down in the grass, and led them to a secret spring in the forest, with which she alone was acquainted. But on their return they found, to their grief, that the unfortunate babe had been killed during their absence, by a serpent. They slew the reptile, and then collecting the remains of the infant, they buried them with funereal honours and proceeded on their way.

The warlike host now appeared before the walls of Thebes, and each leader placed himself before one of the seven gates of the city in readiness for the attack. Eteocles, in conjunction with Creon, had made due preparations to repel the invaders, and had stationed troops, under the command of trusty leaders, to guard each of the gates. Then, according to the practice of the ancients of consulting soothsayers before entering upon any undertaking, the blind old seer Tiresias was sent for, who, after carefully taking the auguries from the flight of birds, declared that all efforts to defend the city would prove unavailing, unless the youngest descendant of the house of Cadmus would offer himself as a voluntary sacrifice for the good of the state.

When Creon heard the words of the seer his first thought was of his favourite son Menœceus, the youngest scion of the royal house, who was present at the interview. He therefore earnestly implored him to leave the city, and to repair for safety to Delphi. But the gallant youth heroically resolved to sacrifice his life for the benefit of his country, and after taking leave of his old father, mounted the city walls, and plunging a dagger into his heart, perished in the sight of the contending hosts.

Adrastus now gave his troops the word of command to storm the city, and they rushed forward to the attack with great valour. The battle raged long and furiously, and after heavy losses on both sides the Argives were routed and put to flight.

After the lapse of some days they reorganized their forces, and again appeared before the gates of Thebes, when Eteocles, grieved to think that there should be such a terrible loss of life on his account, sent a herald into the opposite camp, with a proposition that the fate of the campaign should be decided by single combat between himself and his brother Polynices. The challenge was readily accepted, and in the duel which took place outside the city walls, in the sight of the rival forces, Eteocles and Polynices were both fatally wounded and expired on the field of battle.

Both sides now claimed the day, and the result was that hostilities recommenced, and soon the battle raged with greater fury than ever. But victory at last declared itself for the Thebans. In their flight the Argives lost all their leaders, Adrastus excepted, who owed his safety to the fleetness of his horse Arion.

By the death of the brothers, Creon became once more king of Thebes, and in order to show his abhorrence of the conduct of Polynices in fighting against his country, he strictly forbade any one to bury either his remains or those of his allies. But the faithful Antigone, who had returned to Thebes on the death of her father, could not endure that the body of her brother should remain unburied. She therefore bravely disregarded the orders of the king, and endeavoured to give sepulture to the remains of Polynices.

When Creon discovered that his commands had been set at defiance, he inhumanly condemned the devoted maiden to be entombed alive in a subterranean vault. But retribution was at hand. His son, Hæmon, who was betrothed to Antigone, having contrived to effect an entrance into the vault, was horrified to find that Antigone had hanged herself by her veil. Feeling that life without her would be intolerable, he threw himself in despair on his own sword, and after solemnly invoking the malediction of the gods on the head of his father, expired beside the dead body of his betrothed.

Hardly had the news of the tragic fate of his son reached the king, before another messenger appeared, bearing the tidings that his wife Eurydice, on hearing of the death of Hæmon, had put an end to her existence, and thus the king found himself in his old age both widowed and childless.

Nor did he succeed in the execution of his vindictive designs; for Adrastus, who, after his flight from Thebes, had taken refuge at Athens, induced Theseus to lead an army against the Thebans, to compel them to restore the dead bodies of the Argive warriors to their friends, in order that they might perform due funereal rites in honour of the slain. This undertaking was successfully accomplished, and the remains of the fallen heroes were interred with due honours.

Text:
Myths and Legends of Ancient Greece and Rome
Author: E.M. Berens
Published: 1880

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